CD163 antibody (AA 122-1039)

Catalog No. ABIN7599846

The Rabbit Polyclonal anti-CD163 antibody has been validated for WB, IHC, FACS and ELISA. It is suitable to detect CD163 in samples from Mouse and Rat.

Quick Overview for CD163 antibody (AA 122-1039) (ABIN7599846)

Target: CD163

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Product Details anti-CD163 Antibody

Binding Specificity: AA 122-1039

Purpose: Anti-Cd163 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cd163 Antibody (ABIN7599846). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Cd163 recombinant protein (Position: N122-H1039).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD163

Target: CD163

Alternative Name: Cd163

Background:

Synonyms: Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163, sCD163, CD163, M130

Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Background: CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 150-160 kDa

Gene ID: 93671