Nerve Growth Factor antibody (AA 122-186)

Catalog No. ABIN7599850

This anti-Nerve Growth Factor antibody is a Rabbit Polyclonal antibody detecting Nerve Growth Factor in WB and ELISA. Suitable for Human.

Quick Overview for Nerve Growth Factor antibody (AA 122-186) (ABIN7599850)

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Nerve Growth Factor Antibody

Binding Specificity: AA 122-186

Purpose: Anti-NGF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NGF Antibody Picoband® (ABIN7599850). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NGF recombinant protein (Position: S122-D186).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Breakefield, X. O., Orloff, G., Castiglione, C., Coussens, L., Axelrod, F. B., Ullrich, A. Structural gene for beta-nerve growth factor not defective in familial dysautonomia. Proc. Nat. Acad. Sci. 81: 4213-4216, 1984. 2. Carrier, A., Rosier, M.-F., Guillemot, F., Goguel, A.-F., Pulcini, F., Bernheim, A., Auffray, C., Devignes, M.-D. Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13. Genomics 31: 80-89, 1996. 3. Carvalho, O. P., Thornton, G. K., Hertecant, J., Houlden, H., Nicholas, A. K., Cox, J. J., Rielly, M., Al-Gazali, L., Woods, C. G. A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy. J. Med. Genet. 48: 131-135, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background:

Synonyms: Beta-nerve growth factor, Beta-NGF, NGF, NGFB

Tissue Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Background: Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. It is mapped to 1p13.2. This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

Molecular Weight: 27 kDa

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size