Atp5f1b (AA 123-529) antibody

Catalog No. ABIN7599866

This Rabbit Polyclonal antibody specifically detects in ELISA, WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for Atp5f1b (AA 123-529) antibody (ABIN7599866)

Target: Atp5f1b

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 123-529

Purpose: Anti-ATP5F1B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ATP5F1B Antibody Picoband® (ABIN7599866). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATP5F1B recombinant protein (Position: Q123-S529).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kim, K., Kim, S.-H., Kim, J., Kim, H., Yim, J. Glutathione S-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. J. Biol. Chem. 287: 6628-6641, 2012. 2. Kudoh, J., Minoshima, S., Fukuyama, R., Maekawa, M., Neckelmann, N., Wallace, D. C., Shimizu, Y., Shimizu, N. Assignment of ATP synthase beta subunit (ATPMB) gene to the p13-qter region of human chromosome 12. (Abstract) Cytogenet. Cell Genet. 51: 1026 only, 1989. 3. Martinez, L. O., Jacquet, S., Esteve, J.-P., Rolland, C., Cabezon, E., Champagne, E., Pineau, T., Georgeaud, V., Walker, J. E., Terce, F., Collet, X., Perret, B., Barbaras, R. Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis. Nature 421: 75-79, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Atp5f1b

Background:

Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29

Tissue Specificity: Ubiquitously expressed.

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Molecular Weight: 54 kDa

Gene ID: 506

UniProt: P06576