HTATSF1 antibody (AA 126-333)

Catalog No. ABIN7599887

This Rabbit Polyclonal antibody specifically detects HTATSF1 in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HTATSF1 antibody (AA 126-333) (ABIN7599887)

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HTATSF1 Antibody

Binding Specificity: AA 126-333

Purpose: Anti-HTATSF1 Antibody Picoband®

Characteristics: Anti-HTATSF1 Antibody Picoband® (ABIN7599887). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HTATSF1 recombinant protein (Position: H126-D333). Human HTATSF1 shares 96.2% amino acid (aa) sequence identity with mouse HTATSF1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fong, Y. W., Zhou, Q. Stimulatory effect of splicing factors on transcriptional elongation. Nature 414: 929-933, 2001. 2. Stumpf, A. M. Personal Communication. Baltimore, Md. 10/01/2020. 3. Zhang, Z., Will, C. L., Bertram, K., Dybkov, O., Hartmuth, K., Agafonov, D. E., Hofele, R., Urlaub, H., Kastner, B., Luhrmann, R., Stark, H. Molecular architecture of the human 17S U2 snRNP. Nature 583: 310-313, 2020.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1

Background: HIV Tat-specific factor 1 is a protein that in humans is encoded by the HTATSF1 gene. The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 140 kDa

Gene ID: 27336

UniProt: O43719