RAPGEF2 antibody (AA 1266-1499)

Catalog No. ABIN7599891

The Rabbit Polyclonal anti-RAPGEF2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect RAPGEF2 in samples from Human.

Quick Overview for RAPGEF2 antibody (AA 1266-1499) (ABIN7599891)

Target: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPGEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RAPGEF2 Antibody

Binding Specificity: AA 1266-1499

Purpose: Anti-RAPGEF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAPGEF2 Antibody Picoband® (ABIN7599891). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAPGEF2 recombinant protein (Position: S1266-V1499).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. de Rooij, J., Boenink, N. M., van Triest, M., Cool, R. H., Wittinghofer, A., Bos, J. L. PDZ-GEF1, a guanine nucleotide exchange factor specific for Rap1 and Rap2. J. Biol. Chem. 274: 38125-38130, 1999. 2. Ishiura, H., Doi, K., Mitsui, J., Yoshimura, J., Matsukawa, M. K., Fujiyama, A., Toyoshima, Y., Kakita, A., Takahashi, H., Suzuki, Y., Sugano, S., Qu, W., and 55 others. Expansions of intronic TTTCA and TTTTA repeats in benign adult familial myoclonic epilepsy. Nature Genet. 50: 581-590, 2018. 3. Kawajiri, A., Itoh, N., Fukata, M., Nakagawa, M., Yamaga, M., Iwamatsu, A., Kaibuchi, K. Identification of a novel beta-catenin-interacting protein. Biochem. Biophys. Res. Commun. 273: 712-717, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAPGEF2

Target: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Alternative Name: RAPGEF2

Background:

Synonyms: Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: Rap guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the RAPGEF2 gene. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation.

Molecular Weight: 200-220 kDa

Gene ID: 9693

UniProt: Q9Y4G8

Pathways: Neurotrophin Signaling Pathway