RAPGEF2 antibody (C-Term)

Catalog No. ABIN6243297

The Rabbit Polyclonal anti-RAPGEF2 antibody is suitable to detect RAPGEF2 in samples from Human. It has been validated for WB.

Quick Overview for RAPGEF2 antibody (C-Term) (ABIN6243297)

Target: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPGEF2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB52925

Product Details anti-RAPGEF2 Antibody

Binding Specificity: AA 1464-1498, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RAPGEF2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1464-1498 amino acids from the C-terminal region of human RAPGEF2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for RAPGEF2

Target: RAPGEF2 (Rap Guanine Nucleotide Exchange Factor (GEF) 2 (RAPGEF2))

Alternative Name: RAPGEF2

Background: Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP (PubMed:< a href="http://www.uniprot.org/citations/23800469" target="_blank">23800469, PubMed:< a href="http://www.uniprot.org/citations/10801446" target="_blank">10801446) or not (PubMed:< a href="http://www.uniprot.org/citations/10608844" target="_blank">10608844, PubMed:< a href="http://www.uniprot.org/citations/10548487" target="_blank">10548487, PubMed:< a href="http://www.uniprot.org/citations/11359771" target="_blank">11359771). Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP- induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain- derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.

Molecular Weight: 167417

UniProt: Q9Y4G8

Pathways: Neurotrophin Signaling Pathway