Lamin B2 antibody (AA 128-585)

Catalog No. ABIN7599906

The Rabbit Polyclonal anti-Lamin B2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Lamin B2 in samples from Human and Rat.

Quick Overview for Lamin B2 antibody (AA 128-585) (ABIN7599906)

Target: Lamin B2 (LMNB2)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Lamin B2 Antibody

Binding Specificity: AA 128-585

Purpose: Anti-Lamin B2/LMNB2 Antibody Picoband®

Characteristics: Anti-Lamin B2/LMNB2 Antibody Picoband® (ABIN7599906). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin B2/LMNB2 recombinant protein (Position: L128-E585). Human LMNB2 shares 81.8% amino acid (aa) sequence identity with mouse LMNB2.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Biamonti, G., Giacca, M., Perini, G., Contreas, G., Zentilin, L., Weighardt, F., Guerra, M., Della Valle, G., Saccone, S., Riva, S., Falaschi, A. The gene for a novel human lamin maps at a highly transcribed locus of chromosome 19 which replicates at the onset of S-phase. Molec. Cell. Biol. 12: 3499-3506, 1992. 2. Damiano, J. A., Afawi, Z., Bahlo, M., Mauermann, M., Misk, A., Arsov, T., Oliver, K. L., Dahl, H.-H. M., Shearer, A. E., Smith, R. J. H., Hall, N. E., Mahmood, K., Leventer, R. J., Scheffer, I. E., Muona, M., Lehesjoki, A.-E., Korczyn, A. D., Herrmann, H., Berkovic, S. F., Hildebrand, M. S. Mutation of the nuclear lamin gene LMNB2 in progressive myoclonus epilepsy with early ataxia. Hum. Molec. Genet. 24: 4483-4490, 2015. 3. Gao, J., Li, Y., Fu, X., Luo, X. A Chinese patient with acquired partial lipodystrophy caused by a novel mutation with LMNB2 gene. J. Pediat. Endocr. Metab. 25: 375-377, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: Lamin B2/LMNB2

Background: Lamin B2 is a protein that in humans is encoded by the LMNB2 gene. This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.

Molecular Weight: 72 kDa

Gene ID: 84823

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis