AGO1 antibody (AA 131-171)

Catalog No. ABIN7599960

The Rabbit Polyclonal anti-AGO1 antibody has been validated for WB, ELISA, FACS, ICC and IF. It is suitable to detect AGO1 in samples from Human.

Quick Overview for AGO1 antibody (AA 131-171) (ABIN7599960)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-AGO1 Antibody

Binding Specificity: AA 131-171

Purpose: Anti-AGO1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-AGO1 Antibody Picoband® (ABIN7599960). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AGO1 recombinant protein (Position: K131-R171).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Guo, S., Kemphues, K.J. (1995). "par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed". Cell. 81 (4): 611-620. 2. Hannon, GJ (2002). "RNA interference". Nature.418 (6894): 244-51. 3. Meins F, Jr, Si-Ammour, A, Blevins, T (2005). "RNA silencing systems and their relevance to plant development.". Annual Review of Cell and Developmental Biology. 21 (1): 297-318.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1

Background:

Synonyms: Protein argonaute-1,Argonaute1,hAgo1,Argonaute RISC catalytic component 1,Eukaryotic translation initiation factor 2C 1,eIF-2C 1,eIF2C 1,Putative RNA-binding protein Q99,AGO1,EIF2C1,

Tissue Specificity: Detected in blood plasma (at protein level).

Background: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Molecular Weight: 97 kDa

Gene ID: 26523

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization