NOL9 antibody (AA 131-636)

Catalog No. ABIN7599963

Product Details anti-NOL9 Antibody

Target: NOL9 (Nucleolar Protein 9 (NOL9))

Binding Specificity: AA 131-636

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Anti-NOL9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NOL9 Antibody Picoband® (ABIN7599963). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOL9 recombinant protein (Position: E131-N636). Human NOL9 shares 64.8% amino acid (aa) sequence identity with mouse NOL9.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Bielczyk-Maczynska, E., Lam Hung, L., Ferreira, L., Fleischmann, T., Weis, F., Fernandez-Pevida, A., Harvey, S. A., Wali, N., Warren, A. J., Barroso, I., Stemple, D. L., Cvejic, A. The ribosome biogenesis protein Nol9 is essential for definitive hematopoiesis and pancreas morphogenesis in zebrafish. PLoS Genet. 11: e1005677, 2015. 2. Fujinami, H., Shiraishi, H., Hada, K., Inoue, M., Morisaki, I., Higa, R., Shin, T., Kobayashi, T., Hanada, R., Penninger, J. M., Mimata, H., Hanada, T. CLP1 acts as the main RNA kinase in mice. Biochem. Biophys. Res. Commun. 525: 129-134, 2020. 3. Gordon, J., Pillon, M. C., Stanley, R. E. Nol9 is a spatial regulator for the human ITS2 pre-rRNA endonuclease-kinase complex. J. Molec. Biol. 431: 3771-3786, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NOL9

Target: NOL9 (Nucleolar Protein 9 (NOL9))

Alternative Name: NOL9 (NOL9 Products)

Background:

Synonyms: NOL9, Polynucleotide 5'-hydroxyl-kinase NOL9, EC 2.7.1.-, Nucleolar protein 9

Background: Nucleolar protein 9 is a protein that in humans is encoded by the NOL9 gene. Enables ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity. Involved in maturation of 5.8S rRNA. Located in intermediate filament cytoskeleton and nucleolus.

Molecular Weight: 79 kDa

Gene ID: 79707