PDLIM4 antibody (AA 137-271)

Catalog No. ABIN7599989

This Rabbit Polyclonal antibody specifically detects PDLIM4 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for PDLIM4 antibody (AA 137-271) (ABIN7599989)

Target: PDLIM4 (PDZ and LIM Domain 4 (PDLIM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PDLIM4 Antibody

Binding Specificity: AA 137-271

Purpose: Anti-PDLIM4 Antibody Picoband®

Characteristics: Anti-PDLIM4 Antibody Picoband® (ABIN7599989). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDLIM4 recombinant protein (Position: Y137-D271). Human PDLIM4 shares 91.2% and 89% amino acid (aa) sequence identity with mouse and rat PDLIM4, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bashirova, A. A., Markelov, M. L., Shlykova, T. V., Levshenkova, E. V., Alibaeva, R. A., Frolova, E. I. The human RIL gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts. Gene 210: 239-245, 1998. 2. Boumber, Y. A., Kondo, Y., Chen, X., Shen, L., Gharibyan, V., Konishi, K., Estey, E., Kantarjian, H., Garcia-Manero, G., Issa, J.-P. J. RIL, a LIM gene on 5q31, is silenced by methylation in cancer and sensitizes cancer cells to apoptosis. Cancer Res. 67: 1997-2005, 2007. 3. Kiess, M., Scharm, B., Aguzzi, A., Hajnal, A., Klemenz, R., Schwarte-Waldhoff, I., Schafer, R. Expression of ril, a novel LIM domain gene, is down-regulated in Hras-transformed cells and restored in phenotypic revertants. Oncogene 10: 61-68, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDLIM4

Target: PDLIM4 (PDZ and LIM Domain 4 (PDLIM4))

Alternative Name: PDLIM4

Background: PDZ and LIM domain protein 4 is a protein that in humans is encoded by the PDLIM4 gene. RIL, also known as PDLIM4 is a putative tumor suppressor gene. RIL is transcriptionally repressed by hypermethylation in a variety of tumors including breast and prostate. The difference in methylation status of RIL between normal and cancerous tissues is being investigated as to determine the use of RIL as a potential biomarker. Two isoforms of human RIL one have been identified.

Molecular Weight: 35 kDa

Gene ID: 8572

UniProt: P50479