CASP5 antibody (AA 137-434)

Catalog No. ABIN7599992

This Rabbit Polyclonal antibody specifically detects CASP5 in WB, ELISA, IHC, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CASP5 antibody (AA 137-434) (ABIN7599992)

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-CASP5 Antibody

Binding Specificity: AA 137-434

Purpose: Anti-Caspase-5/CASP5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Caspase-5/CASP5 Antibody Picoband® (ABIN7599992). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Caspase-5/CASP5 recombinant protein (Position: D137-N434).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Munday, N. A., Vaillancourt, J. P., Ali, A., Casano, F. J., Miller, D. K., Molineaux, S. M., Yamin, T.-T., Yu, V. L., Nicholson, D. W. Molecular cloning and pro-apoptotic activity of ICE(rel)II and ICE(rel)III, members of the ICE/CED-3 family of cysteine proteases. J. Biol. Chem. 270: 15870-15876, 1995. 2. Nasir, J., Theilmann, J. L., Vaillancourt, J. P., Munday, N. A., Ali, A., Scherer, S., Beatty, B., Nicholson, D. W., Hayden, M. R. Interleukin-1-beta-converting enzyme (ICE) and related cell death genes ICE(rel)-II and ICE(rel)-III map to the same PAC clone at band 11q22.2-22.3. Mammalian Genome 8: 611-613, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CASP5

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Alternative Name: CASP5

Background:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein, this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.

Molecular Weight: 50 kDa

Gene ID: 838

UniProt: P51878

Pathways: Inflammasome