CD9 antibody (AA 139-192)

Catalog No. ABIN7600001

This Rabbit Polyclonal antibody specifically detects CD9 in FACS, WB, IHC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for CD9 antibody (AA 139-192) (ABIN7600001)

Target: CD9

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CD9 Antibody

Binding Specificity: AA 139-192

Purpose: Anti-CD9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD9 Antibody Picoband® (ABIN7600001). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD9 recombinant protein (Position: Q139-K192).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984). "Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells". Eur J Immunol 13(12): 1008-1013. 2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000). "Severely Reduced Female Fertility in CD9-Deficient Mice". Science 287(5451): 319-321. 3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007). "Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution".Developmental Biology 304 (1): 317-325.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background:

Synonyms: Microtubule-associated protein 2, MAP-2, MAP2

Background: CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli.

Molecular Weight: 22 kDa

Gene ID: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization