SMC5 antibody (AA 14-1089)

Catalog No. ABIN7600007

This Rabbit Polyclonal antibody specifically detects SMC5 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SMC5 antibody (AA 14-1089) (ABIN7600007)

Target: SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SMC5 Antibody

Binding Specificity: AA 14-1089

Purpose: Anti-SMC5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMC5 Antibody Picoband® (ABIN7600007). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMC5 recombinant protein (Position: Q14-Q1089).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Decorsiere, A., Mueller, H., van Breugel, P. C., Abdul, F., Gerossier, L., Beran, R. K., Livingston, C. M., Niu, C., Fletcher, S. P., Hantz, O., Strubin, M. Hepatitis B virus X protein identifies the Smc5/6 complex as a host restriction factor. Nature 531: 386-389, 2016. 2. Kuniba, H., Yoshiura, K., Kondoh, T., Ohashi, H., Kurosawa, K., Tonoki, H., Nagai, T., Okamoto, N., Kato, M., Fukushima, Y., Kaname, T., Naritomi, K., Matsumoto, T., Moriuchi, H., Kishino, T., Kinoshita, A., Miyake, N., Matsumoto, N., Niikawa, N. Molecular karyotyping in 17 patients and mutation screening in 41 patients with Kabuki syndrome. J. Hum. Genet. 54: 304-309, 2009. 3. Nagase, T., Ishikawa, K., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 31-39, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMC5

Target: SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))

Alternative Name: SMC5

Background:

Synonyms: Hepatic leukemia factor, HLF

Tissue Specificity: Highly expressed in liver, lower levels in lung and kidney.

Background: Structural maintenance of chromosomes protein 5 is a protein encoded by the SMC5 gene in human. Predicted to enable ATP binding activity. Involved in several processes, including DNA recombination, cellular senescence, and positive regulation of maintenance of mitotic sister chromatid cohesion. Located in cell junction, chromosome, and nuclear body. Part of Smc5-Smc6 complex.

Molecular Weight: 130 kDa

Gene ID: 23137