SIRT6 antibody (AA 14-355)

Catalog No. ABIN7600019

This Rabbit Polyclonal antibody specifically detects SIRT6 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for SIRT6 antibody (AA 14-355) (ABIN7600019)

Target: SIRT6 (Sirtuin 6 (SIRT6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SIRT6 Antibody

Binding Specificity: AA 14-355

Purpose: Anti-SIRT6 Antibody Picoband®

Characteristics: Anti-SIRT6 Antibody Picoband® (ABIN7600019). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT6 recombinant protein (Position: D14-S355). Human SIRT6 shares 82.7% amino acid (aa) sequence identity with both mouse SIRT6.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Butzow, R., Huhtala, M.-L., Bohn, H., Virtanen, I., Seppala, M. Purification and characterization of placental protein 5. Biochem. Biophys. Res. Commun. 150: 483-490, 1988. Note: Erratum: Biochem. Biophys. Res. Commun. 151: 630-631, 1988. 2. Kisiel, W., Sprecher, C. A., Foster, D. C. Evidence that a second human tissue factor pathway inhibitor (TFPI-2) and human placental protein 5 are equivalent. (Letter) Blood 84: 4384-4385, 1994. 3. Ma, S., Chan, Y. P., Kwan, P. S., Lee, T. K., Yan, M., Tang, K. H., Ling, M. T., Vielkind, J. R., Guan, X.-Y., Chan, K. W. MicroRNA-616 induces androgen-independent growth of prostate cancer cells by suppressing expression of tissue factor pathway inhibitor TFPI-2. Cancer Res. 71: 583-592, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Alternative Name: SIRT6

Background: Sirtuin 6 (SIRT6 or Sirt6) is a stress responsive protein deacetylase and mono-ADP ribosyltransferase enzyme encoded by the SIRT6 gene. This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 45 kDa

Gene ID: 51548

UniProt: Q8N6T7