TRAF1 antibody (AA 14-404)

Catalog No. ABIN7600020

The Rabbit Polyclonal anti-TRAF1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect TRAF1 in samples from Human.

Quick Overview for TRAF1 antibody (AA 14-404) (ABIN7600020)

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TRAF1 Antibody

Binding Specificity: AA 14-404

Purpose: Anti-TRAF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRAF1 Antibody Picoband® (ABIN7600020). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRAF1 recombinant protein (Position: D14-D404).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mosialos, G., Birkenbach, M., Yalamanchili, R., VanArsdale, T., Ware, C., Kieff, E. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 80: 389-399, 1995. 2. Siemienski, K., Peters, N., Scheurich, P., Wajant, H. Organization of the human tumour necrosis factor receptor-associated factor 1 (TRAF1) gene and mapping to chromosome 9q33-34. Gene 195: 35-39, 1997. 3. Tsitsikov, E. N., Laouini, D., Dunn, I. F., Sannikova, T. Y., Davidson, L., Alt, F. W., Geha, R. S. TRAF1 is a negative regulator of TNF signaling: enhanced TNF signaling in TRAF1-deficient mice. Immunity 15: 647-657, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TRAF1

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Alternative Name: TRAF1

Background:

Synonyms: KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, hOT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, KISS1R, AXOR12, GPR54

Tissue Specificity: Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism.

Background: TRAF1 (TNF Receptor-Associated Factor 1), also called EBI6, is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. Siemienski et al. (1997) used fluorescence in situ hybridization to map the TRAF1 gene to 9q33-q34. Mosialos et al. (1995) found that LMP1, the EBV-transforming protein, specifically associates with LAP1 (TRAF3) or EBI6 in B lymphoblasts. LMP1 expression res LAP1 and EBI6 from scattered cytoplasmic structures to LMP1 plasma membrane patches. Both LAP1 and EBI6 associated with the cytoplasmic domain of p80/TNFR2 in vivo.

Molecular Weight: 46 kDa

Gene ID: 7185

UniProt: Q13077

Pathways: NF-kappaB Signaling, Apoptosis, Cell-Cell Junction Organization, Asymmetric Protein Localization