SMAP2 antibody (AA 142-429)

Catalog No. ABIN7600051

Product Details anti-SMAP2 Antibody

Target: SMAP2 (Small ArfGAP2 (SMAP2))

Binding Specificity: AA 142-429

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-SMAP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SMAP2 Antibody Picoband® (ABIN7600051). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMAP2 recombinant protein (Position: K142-K429). Human SMAP2 shares 94.1% amino acid (aa) sequence identity with mouse SMAP2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/22/2016. 2. Kobayashi, N., Kon, S., Henmi, Y., Funaki, T., Satake, M., Tanabe, K. The Arf GTPase-activating protein SMAP1 promotes transferrin receptor endocytosis and interacts with SMAP2. Biochem. Biophys. Res. Commun. 453: 473-479, 2014. 3. Natsume, W., Tanabe, K., Kon, S., Yoshida, N., Watanabe, T., Torii, T., Satake, M. SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network. Molec. Biol. Cell 17: 2592-2603, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMAP2

Target: SMAP2 (Small ArfGAP2 (SMAP2))

Alternative Name: SMAP2 (SMAP2 Products)

Background:

Synonyms: SMAP2, SMAP1L, Stromal membrane-associated protein 2, Stromal membrane-associated protein 1-like

Background: Predicted to enable GTPase activator activity. Located in cytosol and nucleoplasm.

Molecular Weight: 47 kDa

Gene ID: 64744