NADK2 antibody (AA 145-442)

Catalog No. ABIN7600068

The Rabbit Polyclonal anti-NADK2 antibody has been validated for WB, ELISA, FACS, ICC and IF. It is suitable to detect NADK2 in samples from Human, Mouse and Rat.

Quick Overview for NADK2 antibody (AA 145-442) (ABIN7600068)

Target: NADK2 (NAD Kinase 2, Mitochondrial (NADK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NADK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NADK2 Antibody

Binding Specificity: AA 145-442

Purpose: Anti-C5orf33/NADK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-C5orf33/NADK2 Antibody Picoband® (ABIN7600068). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human C5orf33/NADK2 recombinant protein (Position: D145-Q442).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 5/12/2014. 2. Houten, S. M., Denis, S., te Brinke, H., Jongejan, A., van Kampen, A. H. C., Bradley, E. J., Baas, F., Hennekam, R. C. M., Millington, D. S., Young, S. P., Frazier, D. M., Gucsavas-Calikoglu, M., Wanders, R. J. A. Mitochondrial NADP(H) deficiency due to a mutation in NADK2 causes dienoyl-CoA reductase deficiency with hyperlysinemia. Hum. Molec. Genet. 23: 5009-5016, 2014. 3. Ohashi, K., Kawai, S., Murata, K. Identification and characterization of a human mitochondrial NAD kinase. Nature Commun. 3: 1248, 2012. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NADK2

Target: NADK2 (NAD Kinase 2, Mitochondrial (NADK2))

Alternative Name: NADK2

Background:

Synonyms: Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32, UNQ1845, PRO3573

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants.

Molecular Weight: 53 kDa

Gene ID: 133686

UniProt: Q4G0N4