CA9 antibody (AA 146-414)

Catalog No. ABIN7600076

This Rabbit Polyclonal antibody specifically detects CA9 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for CA9 antibody (AA 146-414) (ABIN7600076)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-CA9 Antibody

Binding Specificity: AA 146-414

Purpose: Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Carbonic Anhydrase 9/CA9 Antibody Picoband® (ABIN7600076). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Carbonic Anhydrase 9/CA9 recombinant protein (Position: D146-D414).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Hewett-Emmett, D., Tashian, R. E. Functional diversity, conservation, and convergence in the evolution of the alpha-, beta- and gamma- carbonic anhydrase gene families. Molec. Phylogenet. Evol. 5: 50-77, 1996. 2. Ivanov, S. V., Kuzmin, I., Wei, M.-H., Pack, S., Geil, L., Johnson, B. E., Stanbridge, E. J., Lerman, M. I. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes. Proc. Nat. Acad. Sci. 95: 12596-12601, 1998. 3. Nakagawa, Y., Uemura, H., Hirao, Y., Yoshida, K., Saga, S., Yoshikawa, K. Radiation hybrid mapping of the human MN/CA9 locus to chromosome band 9p12-p13. Genomics 53: 118-119, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CA9

Background:

Synonyms: Myosin-binding protein C, cardiac-type, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC3

Tissue Specificity: Red blood cells.

Background: Carbonic anhydrase IX (CA9/CA IX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Molecular Weight: 54 kDa

Gene ID: 768

UniProt: Q16790