EpCAM antibody (AA 147-189)

Catalog No. ABIN7600081

This anti-EpCAM antibody is a Rabbit Polyclonal antibody detecting EpCAM in IF, IHC and ELISA. Suitable for Mouse and Rat.

Quick Overview for EpCAM antibody (AA 147-189) (ABIN7600081)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-EpCAM Antibody

Binding Specificity: AA 147-189

Purpose: Anti-EpCAM Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EpCAM Antibody Picoband® (ABIN7600081). Tested in ELISA, IF, IHC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse EpCAM recombinant protein (Position: E147-N189).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Litvinov, Sergey, et al. (1994). "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule". The Journal of Cell Biology 125 (2): 437-46. 2. Maetzel, Dorothea, et al. (2009). "Nuclear signalling by tumour-associated antigen EpCAM". Nature Cell Biology 11 (2): 162-71.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Epcam

Background:

Synonyms: Agouti-related protein, Agrp, Agrt, Art

Tissue Specificity: Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Molecular Weight: 87 kDa

Gene ID: 17075