RIPK2 antibody (AA 15-540)

Catalog No. ABIN7600113

The Rabbit Polyclonal anti-RIPK2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect RIPK2 in samples from Human.

Quick Overview for RIPK2 antibody (AA 15-540) (ABIN7600113)

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RIPK2 Antibody

Binding Specificity: AA 15-540

Purpose: Anti-RIP2/RIPK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RIP2/RIPK2 Antibody Picoband® (ABIN7600113). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RIP2/RIPK2 recombinant protein (Position: Y15-M540).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. McCarthy, J. V., Ni, J., Dixit, V. M. RIP2 is a novel NF-kappa-B-activating and cell death-inducing kinase. J. Biol. Chem. 273: 16968-16975, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RIPK2

Target: RIPK2 (Receptor-Interacting Serine-threonine Kinase 2 (RIPK2))

Alternative Name: RIPK2

Background:

Synonyms: Fibrinogen alpha chain, FGA

Tissue Specificity: Detected in blood plasma (at protein level).

Background: RIPK2(Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is anenzymethat in humans is encoded by theRIPK2gene.It has 540-amino acid protein in length.Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein(RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminalcaspase recruitment domain(CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator ofNF-kappa Band inducer of apoptosis in response to various stimuli, CARDIAK(CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.

Molecular Weight: 61 kDa

Gene ID: 8767

UniProt: O43353

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades