ACPP antibody (AA 150-386)

Catalog No. ABIN7600122

The Rabbit Polyclonal anti-ACPP antibody has been validated for WB, IHC, ELISA, ICC, IF and FACS. It is suitable to detect ACPP in samples from Human, Mouse and Rat.

Quick Overview for ACPP antibody (AA 150-386) (ABIN7600122)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ACPP Antibody

Binding Specificity: AA 150-386

Purpose: Anti-PAP/ACP3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PAP/ACP3 Antibody Picoband® (ABIN7600122). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PAP/ACP3 recombinant protein (Position: E150-D386).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fong, L., Brockstedt, D., Benike, C., Breen, J. K., Strang, G., Ruegg, C. L., Engleman, E. G. Dendritic cell-based xenoantigen vaccination for prostate cancer immunotherapy. J. Immun. 167: 7150-7156, 2001. 2. Li, S. S.-L., Sharief, F. S. The prostatic acid phosphatase (ACPP) gene is localized to human chromosome 3q21-q23. Genomics 17: 765-766, 1993. 3. Munch, J., Rucker, E., Standker, L., Adermann, K., Goffinet, C., Schindler, M., Wildum, S., Chinnadurai, R., Rajan, D., Specht, A., Gimenez-Gallego, G., Sanchez, P. C., and 9 others. Semen-derived amyloid fibrils drastically enhance HIV infection. Cell 131: 1059-1071, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACP3

Background:

Synonyms: E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, RING-type E3 ubiquitin transferase TRIM21, Ro (SS-A), Sjoegren syndrome type A antigen, SS-A, Tripartite motif-containing protein 21, TRIM21, RNF81, RO52, SSA1

Tissue Specificity: Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.

Background: Prostatic acid phosphatase (PAP), also prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway.

Molecular Weight: 45-50 kDa

Gene ID: 55

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process