ABI2 antibody (AA 151-400)

Catalog No. ABIN7600125

The Rabbit Polyclonal anti-ABI2 antibody has been validated for WB, ELISA, IHC, FACS, IF and ICC. It is suitable to detect ABI2 in samples from Human and Rat.

Quick Overview for ABI2 antibody (AA 151-400) (ABIN7600125)

Target: ABI2 (Abl-Interactor 2 (ABI2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ABI2 Antibody

Binding Specificity: AA 151-400

Purpose: Anti-ABI2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ABI2 Antibody Picoband® (ABIN7600125). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ABI2 recombinant protein (Position: H151-D400).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Dai, Z., Pendergast, A. M. Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity. Genes Dev. 9: 2569-2582, 1995. 2. Machado, R. D., Pauciulo, M. W., Fretwell, N., Veal, C., Thomson, J. R., Guell, C. V., Aldred, M., Brannon, C. A., the International PPH Consortium, Trembath, R. C., Nichols, W. C. A physical and transcript map based upon refinement of the critical interval for PPH1, a gene for familial primary pulmonary hypertension. Genomics 68: 220-228, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ABI2

Target: ABI2 (Abl-Interactor 2 (ABI2))

Alternative Name: ABI2

Background:

Synonyms: Calretinin, CR, 29 kDa calbindin, CALB2, CAB29

Tissue Specificity: Brain.

Background: Abl interactor 2 also known as Abelson interactor 2 (Abi-2) is a protein that in humans is encoded by the ABI2 gene. Enables several functions, including SH3 domain binding activity, identical protein binding activity, and ubiquitin protein ligase binding activity. Contributes to small GTPase binding activity. Involved in Rac protein signal transduction, positive regulation of cellular component organization, and zonula adherens assembly. Acts upstream of or within peptidyl-tyrosine phosphorylation. Located in several cellular components, including filopodium tip, lamellipodium, and nucleoplasm. Part of SCAR complex. Is active in adherens junction. Colocalizes with actin filament.

Molecular Weight: 56 kDa

Gene ID: 10152