MT-ND5 antibody (AA 154-570)

Catalog No. ABIN7600145

This Rabbit Polyclonal antibody specifically detects MT-ND5 in WB, ELISA, IHC, ICC, FACS and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for MT-ND5 antibody (AA 154-570) (ABIN7600145)

Target: MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MT-ND5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MT-ND5 Antibody

Binding Specificity: AA 154-570

Purpose: Anti-MT-ND5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MT-ND5 Antibody (ABIN7600145). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND5 recombinant protein (Position: L154-Q570).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MT-ND5

Target: MT-ND5 (Mitochondrially Encoded NADH Dehydrogenase 5 (MT-ND5))

Alternative Name: MT-ND5

Background:

Synonyms: Protein Wnt-10a, WNT10A

Tissue Specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

Background: MT-ND5 is a gene of the mitochondrial genome coding for the NADH-ubiquinone oxidoreductase chain 5 protein (ND5). A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 5 (ND5) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encoding for ND5 are implicated in Mitochondrial neurogastrointestinal encephalomyopathy, an autosomal recessive multisystem disorder that results in dramatically elevated levels of circulating thymidine and deoxyuridine.

Molecular Weight: 67 kDa

Gene ID: 4540

UniProt: P03915