RIOK2 antibody (AA 16-283)

Catalog No. ABIN7600169

This Rabbit Polyclonal antibody specifically detects RIOK2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for RIOK2 antibody (AA 16-283) (ABIN7600169)

Target: RIOK2 (RIO Kinase 2 (RIOK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIOK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RIOK2 Antibody

Binding Specificity: AA 16-283

Purpose: Anti-RIOK2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RIOK2 Antibody Picoband® (ABIN7600169). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RIOK2 recombinant protein (Position: D16-L283).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Guderian, G., Peter, C., Wiesner, J., Sickmann, A., Schulze-Osthoff, K., Fischer, U., Grimmler, M. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J. Biol. Chem. 286: 1976-1986, 2011. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/31/2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RIOK2

Target: RIOK2 (RIO Kinase 2 (RIOK2))

Alternative Name: RIOK2

Background:

Synonyms: ATP synthase F (0) complex subunit C1,2,3, mitochondrial, ATP synthase lipid-binding protein, ATP synthase membrane subunit c locus 1,2,3, ATP synthase proteolipid P1, ATP synthase proton-transporting mitochondrial F (0) complex subunit C1,2,3, ATPase protein 9, ATPase subunit c, ATP5MC1,2,3, ATP5G1,2,3

Tissue Specificity: Highly expressed in thymus, uterus and testis. Detected at lower levels in brain, mammary gland, prostate, salivary gland and fetal spleen. In brain, highest expression in thalamus, hippocampus and amygdala. .

Background: RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.

Molecular Weight: 63 kDa

Gene ID: 55781