NEK6 antibody (AA 16-313)

Catalog No. ABIN7600172

This Rabbit Polyclonal antibody specifically detects NEK6 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for NEK6 antibody (AA 16-313) (ABIN7600172)

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NEK6 Antibody

Binding Specificity: AA 16-313

Purpose: Anti-NEK6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NEK6 Antibody Picoband® (ABIN7600172). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NEK6 recombinant protein (Position: N16-T313).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kimura, M., Okano, Y. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenet. Cell Genet. 94: 33-38, 2001. 2. Li, M. Z., Yu, L., Liu, Q., Chu, J. Y., Zhao, S. Y. Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33.3-q34.11 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 271-272, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NEK6

Target: NEK6 (NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6))

Alternative Name: NEK6

Background:

Synonyms: Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Hset1a, Set1/Ash2 histone methyltransferase complex subunit SET1, SETD1A, KIAA0339, KMT2F, SET1, SET1A

Background: Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 10783