RBM15B antibody (AA 164-890)

Catalog No. ABIN7600219

The Rabbit Polyclonal anti-RBM15B antibody has been validated for WB, ELISA, IHC, FACS and IF. It is suitable to detect RBM15B in samples from Human and Rat.

Quick Overview for RBM15B antibody (AA 164-890) (ABIN7600219)

Target: RBM15B (RNA Binding Motif Protein 15B (RBM15B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM15B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-RBM15B Antibody

Binding Specificity: AA 164-890

Purpose: Anti-RBM15B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RBM15B Antibody Picoband® (ABIN7600219). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RBM15B recombinant protein (Position: K164-A890).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hiriart, E., Gruffat, H., Buisson, M., Mikaelian, I., Keppler, S., Meresse, P., Mercher, T., Bernard, O. A., Sergeant, A., Manet, E. Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J. Biol. Chem. 280: 36935-36945, 2005. 2. Patil, D. P., Chen, C.-K., Pickering, B. F., Chow, A., Jackson, C., Guttman, M., Jaffrey, S. R. m6A RNA methylation promotes XIST-mediated transcriptional repression. Nature 537: 369-373, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RBM15B

Target: RBM15B (RNA Binding Motif Protein 15B (RBM15B))

Alternative Name: RBM15B

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome component.

Molecular Weight: 110 kDa

Gene ID: 29890