RBM15B antibody (AA 164-890)

Catalog No. ABIN7600219

Product Details anti-RBM15B Antibody

Target: RBM15B (RNA Binding Motif Protein 15B (RBM15B))

Binding Specificity: AA 164-890

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM15B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Anti-RBM15B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RBM15B Antibody Picoband® (ABIN7600219). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RBM15B recombinant protein (Position: K164-A890).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hiriart, E., Gruffat, H., Buisson, M., Mikaelian, I., Keppler, S., Meresse, P., Mercher, T., Bernard, O. A., Sergeant, A., Manet, E. Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J. Biol. Chem. 280: 36935-36945, 2005. 2. Patil, D. P., Chen, C.-K., Pickering, B. F., Chow, A., Jackson, C., Guttman, M., Jaffrey, S. R. m6A RNA methylation promotes XIST-mediated transcriptional repression. Nature 537: 369-373, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RBM15B

Target: RBM15B (RNA Binding Motif Protein 15B (RBM15B))

Alternative Name: RBM15B (RBM15B Products)

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome component.

Molecular Weight: 110 kDa

Gene ID: 29890