TLN1 antibody (AA 1658-1876)

Catalog No. ABIN7600227

This Rabbit Polyclonal antibody specifically detects TLN1 in WB, ELISA, IHC, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TLN1 antibody (AA 1658-1876) (ABIN7600227)

Target: TLN1 (Talin 1 (TLN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TLN1 Antibody

Binding Specificity: AA 1658-1876

Purpose: Anti-Talin 1/TLN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Talin 1/TLN1 Antibody Picoband® (ABIN7600227). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Talin 1/TLN1 recombinant protein (Position: Q1658-N1876).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Rees, D. J. G., Ades, S. E., Singer, S. J., Hynes, R. O. : Sequence and domain structure of talin. Nature 347: 685-689, 1990. 2. Gilmore, A. P., Ohanian, V., Spurr, N. K., Critchley, D. R. : Localisation of the human gene encoding the cytoskeletal protein talin to chromosome 9p. Hum. Genet. 96: 221-224, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TLN1

Target: TLN1 (Talin 1 (TLN1))

Alternative Name: TLN1

Background:

Synonyms: Talin-1, TLN1, KIAA1027, TLN

Tissue Specificity: Placenta and skin. Predominantly expressed in mammary gland tissue.

Background: Talin-1 is a protein that in humans is encoded by the TLN1 gene. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Molecular Weight: 270 kDa

Gene ID: 7094

UniProt: Q9Y490

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location