NFIA antibody (AA 172-233)

Catalog No. ABIN7600286

This anti-NFIA antibody is a Rabbit Polyclonal antibody detecting NFIA in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NFIA antibody (AA 172-233) (ABIN7600286)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NFIA Antibody

Binding Specificity: AA 172-233

Purpose: Anti-NFIA Antibody Picoband®

Characteristics: Anti-NFIA Antibody Picoband® (ABIN7600286). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NFIA recombinant protein (Position: V172-Q233). Human NFIA shares 100% amino acid (aa) sequence identity with rat NFIA.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Campbell, C. G. N., Wang, H., Hunter, G. W. Interstitial microdeletion of chromosome 1p in two siblings. Am. J. Med. Genet. 111: 289-294, 2002. 2. das Neves, L., Duchala, C. S., Godinho, F., Haxhiu, M. A., Colmenares, C., Macklin, W. B., Campbell, C. E., Butz, K. G., Gronostajski, R. M. Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum. Proc. Nat. Acad. Sci. 96: 11946-11951, 1999. Note: Erratum: Proc. Nat. Acad. Sci. 98: 4276 only, 2001. 3. Deneen, B., Ho, R., Lukaszewicz, A., Hochstim, C. J., Gronostajski, R. M., Anderson, D. J. The transcription factor NFIA controls the onset of gliogenesis in the developing spinal cord. Neuron 52: 953-968, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA

Background: Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 70 kDa

Gene ID: 4774

UniProt: Q12857