SETD3 antibody (AA 173-468)

Catalog No. ABIN7600293

This Rabbit Polyclonal antibody specifically detects SETD3 in WB, ELISA, IHC, FACS, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SETD3 antibody (AA 173-468) (ABIN7600293)

Target: SETD3 (SET Domain Containing 3 (SETD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SETD3 Antibody

Binding Specificity: AA 173-468

Purpose: Anti-SETD3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SETD3 Antibody Picoband® (ABIN7600293). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SETD3 recombinant protein (Position: Q173-R468).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Eom, G. H., Kim, K.-B., Kim, J. H., Kim, J.-Y., Kim, J.-R., Kee, H. J., Kim, D.-W., Choe, N., Park, H.-J., Son, H.-J., Choi, S.-Y., Kook, H., Seo, S.-B. Histone methyltransferase SETD3 regulates muscle differentiation. J. Biol. Chem. 286: 34733-34742, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/27/2014. 3. Wilkinson, A. W., Diep, J., Dai, S., Liu, S., Ooi, Y. S., Song, D., Li, T.-M., Horton, J. R., Zhang, X., Liu, C., Trivedi, D. V., Ruppel, K. M., and 10 others. SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature 565: 372-376, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SETD3

Target: SETD3 (SET Domain Containing 3 (SETD3))

Alternative Name: SETD3

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: SET domain containing 3 (SETD3) is a protein that in humans is encoded by the SETD3 gene. SETD3 is a histone methyltransferase. In mouse muscle cells, Setd3 catalyzes mono- and dimethylation of histone H3 lysine-4 and H3K36 and promotes transcription of genes required for muscle cell differentiation.

Molecular Weight: 60 kDa

Gene ID: 84193

Pathways: Regulation of Muscle Cell Differentiation