POLR1B antibody (AA 18-1135)

Catalog No. ABIN7600313

This anti-POLR1B antibody is a Rabbit Polyclonal antibody detecting POLR1B in WB, ELISA, IHC, FACS, IF and ICC. Suitable for Human.

Quick Overview for POLR1B antibody (AA 18-1135) (ABIN7600313)

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR1B Antibody

Binding Specificity: AA 18-1135

Purpose: Anti-POLR1B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POLR1B Antibody Picoband® (ABIN7600313). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR1B recombinant protein (Position: H18-V1135).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Sanchez, E., Laplace-Builhe, B., Mau-Them, F. T., Richard, E., Goldenberg, A., Toler, T. L., Guignard, T., Gatinois, V., Vincent, M., Blanchet, C., Boland, A., Bihoreau, M. T., and 16 others. POLR1B and neural crest cell anomalies in Treacher Collins syndrome type 4. Genet. Med. 22: 547-556, 2020. 2. Seither, P., Grummt, I. Molecular cloning of RPA2, the gene encoding the second largest subunit of mouse RNA polymerase I. Genomics 37: 135-139, 1996. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 07/06/2020.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: POLR1B

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, Glucose transporter type 9, GLUT-9, SLC2A6, GLUT9

Tissue Specificity: Highly expressed in brain, spleen and peripheral blood leukocytes.

Background: DNA-ed RNA polymerase I subunit RPA2 is an enzyme that in humans is encoded by the POLR1B gene. Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2.

Molecular Weight: 128 kDa

Gene ID: 84172

UniProt: Q9H9Y6