MATK antibody (AA 18-298)

Catalog No. ABIN7600327

The Rabbit Polyclonal anti-MATK antibody has been validated for WB, IHC, ELISA, FACS, ICC and IF. It is suitable to detect MATK in samples from Human, Mouse and Rat.

Quick Overview for MATK antibody (AA 18-298) (ABIN7600327)

Target: MATK (Megakaryocyte-Associated tyrosine Kinase (MATK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MATK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MATK Antibody

Binding Specificity: AA 18-298

Purpose: Anti-MATK Antibody Picoband®

Characteristics: Anti-MATK Antibody Picoband® (ABIN7600327). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MATK recombinant protein (Position: S18-Q298). Human MATK shares 88.5% and 91.4% amino acid (aa) sequence identity with mouse and rat MATK, respectively.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Avraham, S., Jiang, S., Ota, S., Fu, Y., Deng, B., Dowler, L. L., White, R. A., Avraham, H. Structural and functional studies of the intracellular tyrosine kinase MATK gene and its translated product. J. Biol. Chem. 270: 1833-1842, 1995. 2. Bennett, B. D., Cowley, S., Jiang, S., London, R., Deng, B., Grabarek, J., Groopman, J. E., Goeddel, D. V., Avraham, H. Identification and characterization of a novel tyrosine kinase from megakaryocytes. J. Biol. Chem. 269: 1068-1074, 1994. 3. Klages, S., Adam, D., Class, K., Fargnoli, J., Bolen, J. B., Penhallow, R. C. Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family. Proc. Nat. Acad. Sci. 91: 2597-2601, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MATK

Target: MATK (Megakaryocyte-Associated tyrosine Kinase (MATK))

Alternative Name: MATK

Background: Megakaryocyte-associated tyrosine-protein kinase is an enzyme that in humans is encoded by the MATK gene. The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Molecular Weight: 52 kDa

Gene ID: 4145

UniProt: P42679