FBXO7 antibody (AA 18-522)

Catalog No. ABIN7600348

The Rabbit Polyclonal anti-FBXO7 antibody has been validated for WB, ELISA, IHC, IF, IP, ICC and FACS. It is suitable to detect FBXO7 in samples from Human and Rat.

Quick Overview for FBXO7 antibody (AA 18-522) (ABIN7600348)

Target: FBXO7 (F-Box Protein 7 (FBXO7))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-FBXO7 Antibody

Binding Specificity: AA 18-522

Purpose: Anti-FBXO7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FBXO7 Antibody Picoband® (ABIN7600348). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FBXO7 recombinant protein (Position: E18-M522). Human FBXO7 shares 72.6% and 72.1% amino acid (aa) sequence identity with mouse and rat FBXO7, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Cenciarelli, C., Chiaur, D. S., Guardavaccaro, D., Parks, W., Vidal, M., Pagano, M. Identification of a family of human F-box proteins. Curr. Biol. 9: 1177-1179, 1999. 2. Di Fonzo, A., Dekker, M. C. J., Montagna, P., Baruzzi, A., Yonova, E. H., Correia Guedes, L., Szczerbinska, A., Zhao, T., Dubbel-Hulsman, L. O. M., Wouters, C. H., de Graaff, E., Oyen, W. J. G., Simons, E. J., Breedveld, G. J., Oostra, B. A., Horstink, M. W., Bonifati, V. FBXO7 mutations cause autosomal recessive, early-onset parkinsonian-pyramidal syndrome. Neurology 72: 240-245, 2009. 3. Ilyin, G. P., Rialland, M., Pigeon, C., Guguen-Guillouzo, C. cDNA cloning and expression analysis of new members of the mammalian F-box protein family. Genomics 67: 40-47, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FBXO7

Target: FBXO7 (F-Box Protein 7 (FBXO7))

Alternative Name: FBXO7

Background:

Synonyms: FBXO7, FBX7, F-box only protein 7

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined.

Molecular Weight: 59 kDa

Gene ID: 25793

UniProt: Q9Y3I1