H2AFY antibody (AA 180-301)

Catalog No. ABIN7600358

Product Details anti-H2AFY Antibody

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Binding Specificity: AA 180-301

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Purpose: Anti-H2AFY/MACROH2A1 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-H2AFY/MACROH2A1 Antibody Picoband® (ABIN7600358). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human H2AFY/MACROH2A1 recombinant protein (Position: A180-K301). Human MACROH2A1 shares 97.5% amino acid (aa) sequence identity with both mouse and rat MACROH2A1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chakravarthy, S., Gundimella, S. K. Y., Caron, C., Perche, P.-Y., Pehrson, J. R., Khochbin, S., Luger, K. Structural characterization of the histone variant macroH2A. Molec. Cell. Biol. 25: 7616-7624, 2005. 2. Kapoor, A., Goldberg, M. S., Cumberland, L. K., Ratnakumar, K., Segura, M. F., Emanuel, P. O., Menendez, S., Vardabasso, C., LeRoy, G., Vidal, C. I., Polsky, D., Osman, I., Garcia, B. A., Hernando, E., Bernstein, E. The histone variant macroH2A suppresses melanoma progression through regulation of CDK8. Nature 468: 1105-1109, 2010. 3. Kragesteen, B. K., Brancati, F., Digilio, M. C., Mundlos, S., Spielmann, M. H2AFY promoter deletion causes PITX1 endoactivation and Liebenberg syndrome. J. Med. Genet. 56: 246-251, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: MACROH2A1 (H2AFY Products)

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Core histone macro-H2A.1 is a protein that in humans is encoded by the H2AFY gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 39 kDa

Gene ID: 9555

UniProt: O75367