PPWD1 antibody (AA 188-529)

Catalog No. ABIN7600393

The Rabbit Polyclonal anti-PPWD1 antibody has been validated for WB, ELISA, IF, FACS, IHC and ICC. It is suitable to detect PPWD1 in samples from Human, Rat and Mouse.

Quick Overview for PPWD1 antibody (AA 188-529) (ABIN7600393)

Target: PPWD1 (Peptidylprolyl Isomerase Domain and WD Repeat Containing 1 (PPWD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPWD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PPWD1 Antibody

Binding Specificity: AA 188-529

Purpose: Anti-PPWD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PPWD1 Antibody Picoband® (ABIN7600393). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPWD1 recombinant protein (Position: D188-N529).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Davis, T. L., Walker, J. R., Campagna-Slater, V., Finerty, P. J., Jr., Paramanathan, R., Bernstein, G., MacKenzie, F., Tempel, W., Ouyang, H., Lee, W. H., Eisenmesser, E. Z., Dhe-Paganon, S. Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases. PLoS Biol. 8: e1000439, 2010. Note: Electronic Article. 2. Davis, T. L., Walker, J. R., Ouyang, H., MacKenzie, F., Butler-Cole, C., Newman, E. M., Eisenmesser, E. Z., Dhe-Paganon, S. The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1). FEBS J. 275: 2283-2295, 2008. 3. Gross, M. B. Personal Communication. Baltimore, Md. 1/7/2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPWD1

Target: PPWD1 (Peptidylprolyl Isomerase Domain and WD Repeat Containing 1 (PPWD1))

Alternative Name: PPWD1

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

Molecular Weight: 70 kDa

Gene ID: 23398