TREM2 antibody (AA 19-131)

Catalog No. ABIN7600398

This Rabbit Polyclonal antibody specifically detects TREM2 in WB, ELISA and FACS. It exhibits reactivity toward Rat.

Quick Overview for TREM2 antibody (AA 19-131) (ABIN7600398)

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TREM2 Antibody

Binding Specificity: AA 19-131

Purpose: Anti-Trem2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Trem2 Antibody Picoband® (ABIN7600398). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat Trem2 recombinant protein (Position: L19-D131).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Rat
ELISA, 0.1-0.5 μg/mL,
1. Allcock, R. J. N., Barrow, A. D., Forbes, S., Beck, S., Trowsdale, J. The human TREM gene cluster at 6p21.1 encodes both activating and inhibitory single IgV domain receptors and includes NKp44. Europ. J. Immun. 33: 567-577, 2003. 2. Atagi, Y., Liu, C.-C., Painter, M. M., Chen, X.-F., Verbeek, C., Zheng, H., Li, X., Rademakers, R., Kang, S. S., Xu, H., Younkin, S., Das, P., Fryer, J. D., Bu, G. Apolipoprotein E is a ligand for triggering receptor expressed on myeloid cells 2 (TREM2). J. Biol. Chem. 290: 26043-26050, 2015. 3. Bailey, C. C., DeVaux, L. B., Farzan, M. The triggering receptor expressed on myeloid cells 2 binds apolipoprotein E. J. Biol. Chem. 290: 26033-26042, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TREM2

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Alternative Name: Trem2

Background:

Synonyms: Mucin-6, MUC-6, Gastric mucin-6, MUC6

Tissue Specificity: Expressed in the regenerative zone of gastric antrum, gastric body mucosa and gastric incisura mucosa. Expressed in the deeper mucous glands of gastric antrum. Overexpressed in Helicobacter pylori infected gastric epithelium. Highly expressed in duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct.

Background: Triggering receptor expressed on myeloid cells 2 also known as TREM-2 is a protein that in humans is encoded by the TREM2 gene. This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 28 kDa

Gene ID: 301227