SBDS antibody (AA 19-241)

Catalog No. ABIN7600404

This Rabbit Polyclonal antibody specifically detects SBDS in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SBDS antibody (AA 19-241) (ABIN7600404)

Target: SBDS (Shwachman-Bodian-Diamond Syndrome (SBDS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SBDS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SBDS Antibody

Binding Specificity: AA 19-241

Purpose: Anti-SBDS Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SBDS Antibody Picoband® (ABIN7600404). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SBDS recombinant protein (Position: R19-D241).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Austin, K. M., Gupta, M. L., Jr., Coats, S. A., Tulpule, A., Mostoslavsky, G., Balazs, A. B., Mulligan, R. C., Daley, G., Pellman, D., Shimamura, A. Mitotic spindle destabilization and genomic instability in Shwachman-Diamond syndrome. J. Clin. Invest. 118: 1511-1518, 2008. 2. Austin, K. M., Leary, R. J., Shimamura, A. The Shwachman-Diamond SBDS protein localizes to the nucleolus. Blood 106: 1253-1258, 2005. 3. Ball, H. L., Zhang, B., Riches, J. J., Gandhi, R., Li, J., Rommens, J. M., Myers, J. S. Shwachman-Bodian Diamond syndrome is a multi-functional protein implicated in cellular stress responses. Hum. Molec. Genet. 18: 3684-3695, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SBDS

Target: SBDS (Shwachman-Bodian-Diamond Syndrome (SBDS))

Alternative Name: SBDS

Background:

Synonyms: Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12, CASP2, ICH1, NEDD2

Tissue Specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Background: Ribosome maturation protein SBDS is a protein that in humans is encoded by the SBDS gene. This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located.

Molecular Weight: 32 kDa

Gene ID: 51119

UniProt: Q9Y3A5

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly