Thrombospondin 1 antibody (AA 19-268)

Catalog No. ABIN7600407

This Rabbit Polyclonal antibody specifically detects Thrombospondin 1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Thrombospondin 1 antibody (AA 19-268) (ABIN7600407)

Target: Thrombospondin 1 (THBS1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Thrombospondin 1 Antibody

Binding Specificity: AA 19-268

Purpose: Anti-Thrombospondin/THBS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Thrombospondin/THBS1 Antibody Picoband® (ABIN7600407). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Thrombospondin/THBS1 recombinant protein (Position: N19-A268).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Blake SM, Strasser V, Andrade N et al. (October 2008). "Thrombospondin-1 binds to ApoER2 and VLDL receptor and functions in postnatal neuronal migration". The EMBO Journal 27 (22): 3069-80. 2. Lopez-Dee ZP, Chittur SV, Patel B, Stanton R, Wakeley M, Lippert B, Menaker A, Eiche B, Terry R, Gutierrez LS (2012)."Thrombospondin-1 type 1 repeats in a model of inflammatory bowel disease: transcript profile and therapeutic effects". PLoS ONE7 (4): e34590. 3. Simantov R, Silverstein RL (September 2003). "CD36: a critical anti-angiogenic receptor". Frontiers in bioscience : a journal and virtual library 8 (1-3): s874-82.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: THBS1

Background:

Synonyms: Thrombospondin-1,THBS1,TSP, TSP1,

Tissue Specificity: Expressed in natural killer (NK) cells, CD8 (+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).

Background: THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.

Molecular Weight: 129 kDa

Gene ID: 7057

UniProt: P07996

Pathways: Autophagy