SENP1 antibody (AA 19-619)

Catalog No. ABIN7600422

This Rabbit Polyclonal antibody specifically detects SENP1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for SENP1 antibody (AA 19-619) (ABIN7600422)

Target: SENP1 (SUMO1/sentrin Specific Peptidase 1 (SENP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SENP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SENP1 Antibody

Binding Specificity: AA 19-619

Purpose: Anti-SENP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SENP1 Antibody Picoband® (ABIN7600422). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SENP1 recombinant protein (Position: N19-P619).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Veltman, I. M., Vreede, L. A., Cheng, J., Looijenga, L. H. J., Janssen, B., Schoenmakers, E. F. P. M., Yeh, E. T. H., Geurts van Kessel, A. Fusion of the SUMO/sentrin-specific protease 1 gene SENP1 and the embryonic polarity-related mesoderm development gene MESDC2 in a patient with an infantile teratoma and a constitutional t(12,15)(q13,q25). Hum. Molec. Genet. 14: 1955-1963, 2005. 2. Yamaguchi, T., Sharma, P., Athanasiou, M., Kumar, A., Yamada, S., Kuehn, M. R. Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development. Molec. Cell. Biol. 25: 5171-5182, 2005. 3. Yu, L., Ji, W., Zhang, H., Renda, M. J., He, Y., Lin, S., Cheng, E., Chen, H., Krause, D. S., Min, W. SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis. J. Exp. Med. 207: 1183-1195, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SENP1

Target: SENP1 (SUMO1/sentrin Specific Peptidase 1 (SENP1))

Alternative Name: SENP1

Background:

Synonyms: Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1, SENP1

Tissue Specificity: Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

Background: Sentrin-specific protease 1 is a protein that in human is encoded by the SENP1 gene. This gene is mapped to 12q13.11. This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.

Molecular Weight: 73 kDa

Gene ID: 29843

UniProt: Q9P0U3

Pathways: Positive Regulation of Endopeptidase Activity