SENP1 antibody (AA 19-619)

Catalog No. ABIN7600423

The Mouse Monoclonal anti-SENP1 antibody has been validated for WB and FACS. It is suitable to detect SENP1 in samples from Human.

Quick Overview for SENP1 antibody (AA 19-619) (ABIN7600423)

Target: SENP1 (SUMO1/sentrin Specific Peptidase 1 (SENP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SENP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 5F4

Product Details anti-SENP1 Antibody

Binding Specificity: AA 19-619

Purpose: Anti-SENP1 Antibody Picoband® (monoclonal, 5F4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SENP1 Antibody Picoband® (monoclonal, 5F4) (ABIN7600423). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SENP1 recombinant protein (Position: N19-P619).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Veltman, I. M., Vreede, L. A., Cheng, J., Looijenga, L. H. J., Janssen, B., Schoenmakers, E. F. P. M., Yeh, E. T. H., Geurts van Kessel, A. Fusion of the SUMO/sentrin-specific protease 1 gene SENP1 and the embryonic polarity-related mesoderm development gene MESDC2 in a patient with an infantile teratoma and a constitutional t(12,15)(q13,q25). Hum. Molec. Genet. 14: 1955-1963, 2005. 2. Yamaguchi, T., Sharma, P., Athanasiou, M., Kumar, A., Yamada, S., Kuehn, M. R. Mutation of SENP1/SuPr-2 reveals an essential role for desumoylation in mouse development. Molec. Cell. Biol. 25: 5171-5182, 2005. 3. Yu, L., Ji, W., Zhang, H., Renda, M. J., He, Y., Lin, S., Cheng, E., Chen, H., Krause, D. S., Min, W. SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis. J. Exp. Med. 207: 1183-1195, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SENP1

Target: SENP1 (SUMO1/sentrin Specific Peptidase 1 (SENP1))

Alternative Name: SENP1

Background:

Synonyms: Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19

Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Background: Sentrin-specific protease 1 is a protein that in human is encoded by the SENP1 gene. This gene is mapped to 12q13.11. This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.

Molecular Weight: 73 kDa

Gene ID: 29843

UniProt: Q9P0U3

Pathways: Positive Regulation of Endopeptidase Activity