CIAO3 (AA 196-476) antibody

Catalog No. ABIN7600446

This Rabbit Polyclonal antibody specifically detects in ELISA, WB and FACS. It exhibits reactivity toward Human.

Quick Overview for CIAO3 (AA 196-476) antibody (ABIN7600446)

Target: CIAO3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 196-476

Purpose: Anti-NARFL/CIAO3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NARFL-CIAO3 Antibody Picoband® (ABIN7600446). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NARFL/CIAO3 recombinant protein (Position: Y196-W476). Human NARFL/CIAO3 shares 88.3% and 86.8% amino acid (aa) sequence identity with mouse and rat NARFL/CIAO3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/19/2011. 2. Huang, J., Song, D., Flores, A., Zhao, Q., Mooney, S. M., Shaw, L. M., Lee, F. S. IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1-alpha activity. Biochem. J. 401: 341-352, 2007. 3. Song, D., Lee, F. S. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J. Biol. Chem. 286: 15797-15805, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: CIAO3

Background:

Synonyms: CIAO3, NARFL, PRN, Cytosolic iron-sulfur assembly component 3, Cytosolic Fe-S cluster assembly factor NARFL, Iron-only hydrogenase-like protein 1, IOP1, Nuclear prelamin A recognition factor-like protein, Protein related to Narf

Background: Predicted to enable 4 iron, 4 sulfur cluster binding activity and metal ion binding activity. Involved in several processes, including intracellular oxygen homeostasis, iron-sulfur cluster assembly, and response to hypoxia. Part of cytosolic [4Fe-4S] assembly targeting complex.

Molecular Weight: 53 kDa

Gene ID: 64428

UniProt: Q9H6Q4