PPFIA1 antibody (AA 198-1202)

Catalog No. ABIN7600454

This anti-PPFIA1 antibody is a Rabbit Polyclonal antibody detecting PPFIA1 in WB, ELISA, IHC, IF and ICC. Suitable for Human.

Quick Overview for PPFIA1 antibody (AA 198-1202) (ABIN7600454)

Target: PPFIA1 (Protein Tyrosine Phosphatase F Interacting Protein 1 (PPFIA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPFIA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PPFIA1 Antibody

Binding Specificity: AA 198-1202

Purpose: Anti-PPFIA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PPFIA1 Antibody Picoband® (ABIN7600454). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPFIA1 recombinant protein (Position: A198-C1202).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hoogenraad, C. C., Feliu-Mojer, M. I., Spangler, S. A., Milstein, A. D., Dunah, A. W., Hung, A. Y., Sheng, M. Liprin-alpha-1 degradation by calcium/calmodulin-dependent protein kinase II regulates LAR receptor tyrosine phosphatase distribution and dendrite development. Dev. Cell 12: 587-602, 2007. [PubMed: 17419996, related citations] [Full Text] 2. Streuli, M. The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LAR-interacting protein co-localize at focal adhesions. EMBO J. 14: 2827-2838, 1995. 3. Serra-Pages, C., Medley, Q. G., Tang, M., Hart, A., Streuli, M. Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins. J. Biol. Chem. 273: 15611-15620, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPFIA1

Target: PPFIA1 (Protein Tyrosine Phosphatase F Interacting Protein 1 (PPFIA1))

Alternative Name: PPFIA1

Background:

Synonyms: Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38, AHSA1, C14orf3, HSPC322

Tissue Specificity: Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.

Background: Liprin-alpha-1 is a protein that in humans is encoded by the PPFIA1 gene. The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 160 kDa

Gene ID: 8500

UniProt: Q13136