NCBP2 antibody (AA 2-102)

Catalog No. ABIN7600463

This anti-NCBP2 antibody is a Rabbit Polyclonal antibody detecting NCBP2 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NCBP2 antibody (AA 2-102) (ABIN7600463)

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NCBP2 Antibody

Binding Specificity: AA 2-102

Purpose: Anti-CBP20/NCBP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CBP20/NCBP2 Antibody Picoband® (ABIN7600463). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CBP20/NCBP2 recombinant protein (Position: S2-N102).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gebhardt, A., Habjan, M., Benda, C., Meiler, A., Haas, D. A., Hein, M. Y., Mann, A., Mann, M., Habermann, B., Pichlmair, A. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nature Commun. 6: 8192, 2015. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 10/28/2015. 3. Izaurralde, E., Lewis, J., Gamberi, C., Jarmolowski, A., McGuigan, C., Mattaj, I. W. A cap-binding protein complex mediating U snRNA export. Nature 376: 709-712, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Alternative Name: NCBP2

Background:

Synonyms: BMP and activin membrane-bound inhibitor homolog, Non-metastatic gene A protein, Putative transmembrane protein, NMA, BAMBI, NMA

Tissue Specificity: Expressed in adult liver.

Background: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 18 kDa

Gene ID: 22916

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process