STAP1 antibody (AA 2-282)

Catalog No. ABIN7600485

This Rabbit Polyclonal antibody specifically detects STAP1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for STAP1 antibody (AA 2-282) (ABIN7600485)

Target: STAP1 (Signal Transducing Adaptor Family Member 1 (STAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-STAP1 Antibody

Binding Specificity: AA 2-282

Purpose: Anti-STAP-1/STAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-STAP-1/STAP1 Antibody Picoband® (ABIN7600485). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human STAP-1/STAP1 recombinant protein (Position: M2-E282).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 8/11/2011. 2. Ohya, K., Kajigaya, S., Kitanaka, A., Yoshida, K., Miyazato, A., Yamashita, Y., Yamanaka, T., Ikeda, U., Shimada, K., Ozawa, K., Mano, H. Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase. Proc. Nat. Acad. Sci. 96: 11976-11981, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for STAP1

Target: STAP1 (Signal Transducing Adaptor Family Member 1 (STAP1))

Alternative Name: STAP1

Background:

Synonyms: Tudor domain-containing protein 3, TDRD3

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Signal-transducing adaptor protein 1 is a protein that in humans is encoded by the STAP1 gene. The protein encoded by this gene contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. This protein is a substrate of tyrosine-protein kinase Tec, and its interaction with tyrosine-protein kinase Tec is phosphorylation-dependent. This protein is thought to participate in a positive feedback loop by upregulating the activity of tyrosine-protein kinase Tec. Variants of this gene have been associated with autosomal-dominant hypercholesterolemia (ADH), which is characterized by elevated low-density lipoprotein cholesterol levels and in increased risk of coronary vascular disease. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36 kDa

Gene ID: 26228