MPST antibody (AA 2-297)

Catalog No. ABIN7600487

This anti-MPST antibody is a Rabbit Polyclonal antibody detecting MPST in WB, ELISA, FACS, ICC and IF. Suitable for Human.

Quick Overview for MPST antibody (AA 2-297) (ABIN7600487)

Target: MPST (Mercaptopyruvate Sulfurtransferase (MPST))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-MPST Antibody

Binding Specificity: AA 2-297

Purpose: Anti-MPST Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MPST Antibody Picoband® (ABIN7600487). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MPST recombinant protein (Position: A2-H297).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aita, N., Ishii, K., Akamatsu, Y., Ogasawara, Y., Tanabe, S. Cloning and expression of human liver rhodanese cDNA. Biochem. Biophys. Res. Commun. 231: 56-60, 1997. 2. Billaut-Laden, I., Rat, E., Allorge, D., Crunelle-Thibaut, A., Cauffiez, C., Chevalier, D., Lo-Guidice, J.-M., Broly, F. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. Toxicol. Lett. 165: 101-111, 2006. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 12/8/2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MPST

Target: MPST (Mercaptopyruvate Sulfurtransferase (MPST))

Alternative Name: MPST

Background:

Synonyms: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.

Molecular Weight: 33 kDa

Gene ID: 4357

UniProt: P25325