TRIM63 antibody (AA 2-329)

Catalog No. ABIN7600489

The Rabbit Polyclonal anti-TRIM63 antibody has been validated for WB and ELISA. It is suitable to detect TRIM63 in samples from Human and Monkey.

Quick Overview for TRIM63 antibody (AA 2-329) (ABIN7600489)

Target: TRIM63 (Tripartite Motif Containing 63 (TRIM63))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM63 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TRIM63 Antibody

Binding Specificity: AA 2-329

Purpose: Anti-MURF1/TRIM63 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MURF1/TRIM63 Antibody Picoband® (ABIN7600489). Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Monkey
ELISA, 0.1-0.5 μg/mL, -
1. Bodine, S. C., Latres, E., Baumhueter, S., Lai, V. K.-M., Nunez, L., Clarke, B. A., Poueymirou, W. T., Panaro, F. J., E., Dharmarajan, K., Pan, Z.-Q., Valenzuela, D. M., DeChiara, T. M., Stitt, T. N., Yancopoulos, G. D., Glass, D. J. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 294: 1704-1708, 2001. 2. Cai, D., Frantz, J. D., Tawa, N. E., Jr., Melendez, P. A., Oh, B.-C., Lidov, H. G. W., Hasselgren, P.-O., Frontera, W. R., Lee, J., Glass, D. J., Shoelson, S. E. IKK-beta/NF-kappa-B activation causes severe muscle wasting in mice. Cell 119: 285-298, 2004. 3. Centner, T., Yano, J., Kimura, E., McElhinny, A. S., Pelin, K., Witt, C. C., Bang, M.-L., Trombitas, K., Granzier, H., Gregorio, C. C., Sorimachi, H., Labeit, S. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J. Molec. Biol. 306: 717-726, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TRIM63

Target: TRIM63 (Tripartite Motif Containing 63 (TRIM63))

Alternative Name: TRIM63

Background:

Synonyms: Tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6, TRIM6, RNF89

Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Background: E3 ubiquitin-protein ligase TRIM63 is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.

Molecular Weight: 40 kDa

Gene ID: 84676

UniProt: Q969Q1