CD83 antibody (AA 20-144)

Catalog No. ABIN7600519

The Rabbit Polyclonal anti-CD83 antibody has been validated for FACS, WB and ELISA. It is suitable to detect CD83 in samples from Human.

Quick Overview for CD83 antibody (AA 20-144) (ABIN7600519)

Target: CD83

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD83 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-CD83 Antibody

Binding Specificity: AA 20-144

Purpose: Anti-CD83 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD83 Antibody Picoband® (ABIN7600519). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD83 recombinant protein (Position: T20-E144).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Berchtold, S., Muhl-Zurbes, P., Heufler, C., Winklehner, P., Schuler, G., Steinkasserer, A. Cloning, recombinant expression and biochemical characterization of the murine CD83 Molecule which is specifically upregulated during dendritic cell maturation. FEBS Lett. 461: 211-216, 1999. 2. Fujimoto, Y., Tu, L., Miller, A. S., Bock, C., Fujimoto, M., Doyle, C., Steeber, D. A., Tedder, T. F. CD83 expression influences CD4+ T cell development in the thymus. Cell 108: 755-767, 2002. 3. Kozlow, E. J., Wilson, G. L., Fox, C. H., Kehrl, J. H. Subtractive cDNA cloning of a novel member of the Ig gene superfamily expressed at high levels in activated B lymphocytes. Blood 81: 454-461, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD83

Target: CD83

Alternative Name: CD83

Background:

Synonyms: Importin subunit alpha-1,Karyopherin subunit alpha-2,RAG cohort protein 1,SRP1-alpha,KPNA2,RCH1, SRP1,

Tissue Specificity: Expressed ubiquitously.

Background: CD83 (Cluster of Differentiation 83) is a human protein encoded by the CD83 gene. The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 9308

UniProt: Q01151