Histone H1 antibody (AA 20-159)

Catalog No. ABIN7600521

This Rabbit Polyclonal antibody specifically detects Histone H1 in WB, ELISA, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H1 antibody (AA 20-159) (ABIN7600521)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Histone H1 Antibody

Binding Specificity: AA 20-159

Purpose: Anti-Histone H1.0/H1F0 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Histone H1.0/H1F0 Antibody Picoband® (ABIN7600521). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159)

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Doenecke D, Tönjes R (February 1986). "Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene". Journal of Molecular Biology. 187 (3): 461-4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993). "All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6". Genomics. 16 (3): 649-54.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Alternative Name: H1F0

Background:

Synonyms: Histone H1.0, Histone H1', Histone H1 (0), H1F0, H1FV

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: H1 histone family, member 0?is a member of the?histone?family of nuclear?proteins?which are a component of?chromatin. In humans, this protein is encoded by the?H1F0?gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Molecular Weight: 24 kDa

Gene ID: 3005

UniProt: P07305