Histone H1 antibody (AA 20-159)

Catalog No. ABIN7600522

The Mouse Monoclonal anti-Histone H1 antibody has been validated for WB, IHC and FACS. It is suitable to detect Histone H1 in samples from Human and Mouse.

Quick Overview for Histone H1 antibody (AA 20-159) (ABIN7600522)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5I3E6

Product Details anti-Histone H1 Antibody

Binding Specificity: AA 20-159

Purpose: Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) (ABIN7600522). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Doenecke D, Tönjes R (February 1986). "Differential distribution of lysine and arginine residues in the closely related histones H1 and H5. Analysis of a human H1 gene". Journal of Molecular Biology. 187 (3): 461-4. 2. Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik KH, Doenecke D (June 1993). "All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6". Genomics. 16 (3): 649-54.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Alternative Name: H1F0

Background:

Synonyms: T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: H1 histone family, member 0is a member of thehistonefamily of nuclearproteinswhich are a component ofchromatin. In humans, this protein is encoded by theH1F0gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Molecular Weight: 24 kDa

Gene ID: 3005

UniProt: P07305