NSFL1C antibody (AA 20-291)

Catalog No. ABIN7600537

The Rabbit Polyclonal anti-NSFL1C antibody has been validated for WB, IF, ELISA, FACS and ICC. It is suitable to detect NSFL1C in samples from Human, Mouse and Rat.

Quick Overview for NSFL1C antibody (AA 20-291) (ABIN7600537)

Target: NSFL1C (NSFL1 (p97) Cofactor (p47) (NSFL1C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSFL1C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NSFL1C Antibody

Binding Specificity: AA 20-291

Purpose: Anti-NSFL1C Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NSFL1C Antibody Picoband® (ABIN7600537). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSFL1C recombinant protein (Position: E20-E291).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kondo, H., Rabouille, C., Newman, R., Levine, T. P., Pappin, D., Freemont, P., Warren, G. p47 is a cofactor for p97-mediated membrane fusion. Nature 388: 75-78, 1997. 2. Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., and 9 others. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10: 1546-1560, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSFL1C

Target: NSFL1C (NSFL1 (p97) Cofactor (p47) (NSFL1C))

Alternative Name: NSFL1C

Background:

Synonyms: SRC kinase signaling inhibitor 1, SNAP-25-interacting protein, SNIP, p130Cas-associated protein, p140Cap, SRCIN1, KIAA1684, P140

Tissue Specificity: Expressed in some primary breast carcinomas where its presence is significantly associated with increased tumor size. Not detected in normal breast tissue.

Background: NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene. N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Molecular Weight: 41 kDa

Gene ID: 55968