CD1d antibody (AA 20-295)

Catalog No. ABIN7600538

This anti-CD1d antibody is a Rabbit Polyclonal antibody detecting CD1d in FACS, WB and ELISA. Suitable for Human and Rat.

Quick Overview for CD1d antibody (AA 20-295) (ABIN7600538)

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD1d antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Product Details anti-CD1d Antibody

Binding Specificity: AA 20-295

Purpose: Anti-CD1D Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD1D Antibody Picoband® (ABIN7600538). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD1D recombinant protein (Position: E20-W295).

Isotype: IgG

Application Details

Application Notes: "Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
"1. Jayawardena-Wolf, J., Benlagha, K., Chiu, Y.-H., Mehr, R., Bendelac, A. CD1d endosomal trafficking is independently regulated by an intrinsic CD1d-encoded tyrosine motif and by the invariant chain.Immunity 15: 897-908, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD1d

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Alternative Name: CD1D

Background:

Synonyms: Antigen-presenting glycoprotein CD1d; R3G1; CD1d; CD1D

Tissue Specificity: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Background: CD1D (CD1d molecule) also known as R3G1, Antigen-presenting glycoprotein CD1d, R3G1, CD1D, is the sole group-2 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. Expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production. Jayawardena-Wolf et al. described 2 different pathways of Cd1d trafficking to endosomal compartments in mouse cells. A tyrosine-based motif governs recycling between the plasma membrane and the endosome, while Cd1d associates, like major histocompatibility complex class II antigens, with the invariant chain, or II, in the endoplasmic reticulum. Both pathways enhance antigen presentation to Cd1d-restricted natural killer T cells.

Molecular Weight: 55 kDa

Gene ID: 912

UniProt: P15813

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process